Characterization of immortalized human islet stromal cells reveals a MSC-like profile with pancreatic features.

ABSTRACT:

Background

Mesenchymal stromal cells (MSCs) represent an interesting tool to improve pancreatic islet transplantation. They have immunomodulatory properties and secrete supportive proteins. However, the functional properties of MSCs vary according to many factors such as donor characteristics, tissue origin, or isolation methods. To counteract this heterogeneity, we aimed to immortalize and characterize adherent cells derived from human pancreatic islets (hISCs), using phenotypic, transcriptomic, and functional analysis.

Methods

Adherent cells derived from human islets in culture were infected with a hTERT retrovirus vector and then characterized by microarray hybridization, flow cytometry analysis, and immunofluorescence assays. Osteogenic, adipogenic, and chondrogenic diff

SUBMITTER: Villard O 

PROVIDER: S-EPMC7165390 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature