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Transcriptional oncogenomic hot spots in Barrett's adenocarcinomas: serial analysis of gene expression.

ABSTRACT: Serial analysis of gene expression (SAGE) provides quantitative and comprehensive expression profiling in a given cell population. In our efforts to define gene expression alterations in Barrett's-related adenocarcinomas (BA), we produced eight SAGE libraries and obtained a total of 457,894 expressed tags with 32,035 (6.9%) accounting for singleton tags. The tumor samples produced an average of 71,804 tags per library, whereas normal samples produced an average of 42,669 tags per library. Our libraries contained 67,200 unique tags representing 16,040 known gene symbols. Five hundred and sixty-eight unique tags were differentially expressed between BAs and normal tissue samples (at least twofold; P

SUBMITTER: Razvi MH

PROVIDER: S-EPMC7165894 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature

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Transcriptional oncogenomic hot spots in Barrett's adenocarcinomas: serial analysis of gene expression.

Razvi Mohammad H MH Peng Dunfa D Dar Altaf A AA Powell Steven M SM Frierson Henry F HF Moskaluk Christopher A CA Washington Kay K El-Rifai Wael W

Genes, chromosomes & cancer 20071001 10

Serial analysis of gene expression (SAGE) provides quantitative and comprehensive expression profiling in a given cell population. In our efforts to define gene expression alterations in Barrett's-related adenocarcinomas (BA), we produced eight SAGE libraries and obtained a total of 457,894 expressed tags with 32,035 (6.9%) accounting for singleton tags. The tumor samples produced an average of 71,804 tags per library, whereas normal samples produced an average of 42,669 tags per library. Our li ...[more]

PMID: 17636545

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