Isolation and identification of flavonoid-producing endophytic fungi from medicinal plant Conyza blinii H.Lev that exhibit higher antioxidant and antibacterial activities.

ABSTRACT:

Background

Conyza blinii H. Lév is a medicinal plant that has a variety of pharmacological activities, but its study is at a standstill due to the shortage of resources.

Method

This study utilized the surface sterilization method to isolate endophytic fungi, and they were preliminarily identified by morphology. Flavonoid-producing strains were screened by NaNO₂-Al(NO)₃ colorimetry and further identified by the ITS sequence. Additionally, we used five antioxidant assays (DPPH, Hydroxyl radical, ABTS, FRAP and T-AOC assays) to systematically evaluate the antioxidant capacity of total flavonoids , and we also determined their antibacterial activity.

Results

In this study, 21 endophytic fungi were isolated from wild Conyza blinii H. Lév

SUBMITTER: Tang Z 

PROVIDER: S-EPMC7166047 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature