Cryoelectron Microscopy Structure of a Yeast Centromeric Nucleosome at 2.7 A Resolution.

ABSTRACT: Kinetochores mediate chromosome segregation during cell division. They assemble on centromeric nucleosomes and capture spindle microtubules. In budding yeast, a kinetochore links a single nucleosome, containing the histone variant Cse4^CENP-A instead of H3, with a single microtubule. Conservation of most kinetochore components from yeast to metazoans suggests that the yeast kinetochore represents a module of the more complex metazoan arrangements. We describe here a streamlined protocol for reconstituting a yeast centromeric nucleosome and a systematic exploration of cryo-grid preparation. These developments allowed us to obtain a high-resolution cryoelectron microscopy reconstruction. As suggested by previous work, fewer base pairs are in tight association with the histone octam

SUBMITTER: Migl D 

PROVIDER: S-EPMC7166091 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature