Expression profile of genes involved in pathogenesis of pediatric Crohn's disease.

ABSTRACT:

Background and aim

Expression profiling of genes specific to pediatric Crohn's Disease (CD) patients was performed to elucidate the molecular mechanisms underlying disease cause and pathogenesis at disease onset.

Methods

We used suppressive subtractive hybridization (SSH) and differential screening analysis to profile the mRNA expression patterns of children with CD and age- and sex-matched controls without inflammatory bowel disease (IBD).

Results

Sequence analysis of 1000 clones enriched by SSH identified 75 functionally annotated human genes, represented by 430 clones. The 75 genes have potential involvement in gene networks, such as antigen presentation, inflammation, infection mechanism, connective tissue development, cell cycle and cancer. Twenty-eight genes wer

SUBMITTER: Sim WH 

PROVIDER: S-EPMC7167032 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature