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Identification of group B rotavirus as an etiological agent in the gastroenteritis outbreak in Maharashtra, India.

ABSTRACT: Acute gastroenteritis outbreak occurred at Pargaon, Maharashtra, India in 1789 cases with an attack rate of 32.5% between November to December 2015. The stool specimens (n = 32) were investigated for different enteric viral agents using conventional methods. Transmission electron microscopy and RNA polyacrylamide gel electrophoresis respectively identified morphologically distinct rotavirus particles in 28% and RNA migration pattern of Group B Rotavirus (GBR) in 72% of the specimens. Reverse transcription polymerase chain reaction and nucleotide sequencing confirmed presence of GBR in 97% of the samples analyzed. The predominance of GBR infections and absence or insignificant presence of other agents confirmed GBR as an etiological agent of the gastroenteritis outbreak occurred in Maharashtra, India.

SUBMITTER: Joshi MS

PROVIDER: S-EPMC7167091 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Identification of group B rotavirus as an etiological agent in the gastroenteritis outbreak in Maharashtra, India.

Joshi Madhuri S MS Ganorkar Nital N NN Ranshing Sujata S SS Basu Atanu A Chavan Nutan A NA Gopalkrishna Varanasi V

Journal of medical virology 20170811 12

Acute gastroenteritis outbreak occurred at Pargaon, Maharashtra, India in 1789 cases with an attack rate of 32.5% between November to December 2015. The stool specimens (n = 32) were investigated for different enteric viral agents using conventional methods. Transmission electron microscopy and RNA polyacrylamide gel electrophoresis respectively identified morphologically distinct rotavirus particles in 28% and RNA migration pattern of Group B Rotavirus (GBR) in 72% of the specimens. Reverse tra ...[more]

PMID: 28710858

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