Robust production of uniform human cerebral organoids from pluripotent stem cells.

ABSTRACT: Human cerebral organoid (hCO) models offer the opportunity to understand fundamental processes underlying human-specific cortical development and pathophysiology in an experimentally tractable system. Although diverse methods to generate brain organoids have been developed, a major challenge has been the production of organoids with reproducible cell type heterogeneity and macroscopic morphology. Here, we have directly addressed this problem by establishing a robust production pipeline to generate morphologically consistent hCOs and achieve a success rate of >80%. These hCOs include both a radial glial stem cell compartment and electrophysiologically competent mature neurons. Moreover, we show using immunofluorescence microscopy and single-cell profiling that individual organoids display r

SUBMITTER: Sivitilli AA 

PROVIDER: S-EPMC7167289 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature