The CtrA Regulon of Rhodobacter sphaeroides Favors Adaptation to a Particular Lifestyle.

ABSTRACT: Activation of the two-component system formed by CckA, ChpT, and CtrA (kinase, phosphotransferase, and response regulator, respectively) in Rhodobacter sphaeroides does not occur under the growth conditions commonly used in the laboratory. However, it is possible to isolate a gain-of-function mutant in CckA that turns the system on. Using massive parallel transcriptome sequencing (RNA-seq), we identified 321 genes that are differentially regulated by CtrA. From these genes, 239 were positively controlled and 82 were negatively regulated. Genes encoding the Fla2 polar flagella and gas vesicle proteins are strongly activated by CtrA. Genes involved in stress responses as well as several transcriptional factors are also positively controlled, whereas the photosynthetic and CO₂

SUBMITTER: Hernandez-Valle J 

PROVIDER: S-EPMC7167476 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature