Nanoparticle mediated increased insulin-like growth factor 1 expression enhances human placenta syncytium function.

ABSTRACT:

Introduction

Placental dysfunction is an underlying cause of many major obstetric diseases and treatment options for complications like fetal growth restriction (FGR) are limited .We previously demonstrated nanoparticle delivery of the human insulin-like growth factor 1 (hIGF1) transgene under control of the trophoblast-specific PLAC1 promoter maintains normal fetal growth in a surgically-induced FGR mouse model. However, uptake by human placental syncytiotrophoblast has yet to be determined.

Methods

An ex vivo human placenta perfusion model, term placenta villous fragments, and other in vitro syncytiotrophoblast models were used to determine nanoparticle uptake, transgene expression, and functional responses under oxidative stress conditions.

Results

In the ex vivo p

SUBMITTER: Wilson RL 

PROVIDER: S-EPMC7167609 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature