Transcriptome analysis and functional characterization of cerebral organoids in bipolar disorder.

ABSTRACT:

Background

Reprogramming human induced pluripotent stem cells (iPSCs) from somatic cells and generating three-dimensional brain organoids from these iPSCs provide access to live human neuronal tissue with disease-specific genetic backgrounds.

Methods

Cerebral organoids were generated from iPSCs of eight bipolar disorder (BPI) patients and eight healthy control individuals. RNA-seq experiments were undertaken using RNA isolated from the cerebral organoids. Functional activity in the cerebral organoids was studied using microelectrode arrays.

Results

RNA-seq data comparing gene expression profiles in the cerebral organoids showed downregulation of pathways involved in cell adhesion, neurodevelopment, and synaptic biology in bipolar disorder along with upregulation of ge

SUBMITTER: Kathuria A 

PROVIDER: S-EPMC7168850 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature