Sperm Head-Tail Linkage Requires Restriction of Pericentriolar Material to the Proximal Centriole End.

ABSTRACT: The centriole, or basal body, is the center of attachment between the sperm head and tail. While the distal end of the centriole templates the cilia, the proximal end associates with the nucleus. Using Drosophila, we identify a centriole-centric mechanism that ensures proper proximal end docking to the nucleus. This mechanism relies on the restriction of pericentrin-like protein (PLP) and the pericentriolar material (PCM) to the proximal end of the centriole. PLP is restricted proximally by limiting its mRNA and protein to the earliest stages of centriole elongation. Ectopic positioning of PLP to more distal portions of the centriole is sufficient to redistribute PCM and microtubules along the entire centriole length. This results in erroneous, lateral centriole docking to the nucleus, lea

SUBMITTER: Galletta BJ 

PROVIDER: S-EPMC7170007 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature