Ontology highlight
ABSTRACT:
SUBMITTER: Jia M
PROVIDER: S-EPMC7170024 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Jia Mengxuan M Sen Sambuddha S Wachnowsky Christine C Fidai Insiya I Cowan James A JA Wysocki Vicki H VH
Angewandte Chemie (International ed. in English) 20200303 17
Many iron-sulfur proteins involved in cluster trafficking form [2Fe-2S]-cluster-bridged complexes that are often challenging to characterize because of the inherent instability of the cluster at the interface. Herein, we illustrate the use of fast, online buffer exchange coupled to a native mass spectrometry (OBE nMS) method to characterize [2Fe-2S]-cluster-bridged proteins and their transient cluster-transfer intermediates. The use of this mechanistic and protein-characterization tool is demons ...[more]