Incompatible erythrocyte transfusion with lipopolysaccharide induces acute lung injury in a novel rat model.

ABSTRACT: Acute transfusion reactions can manifest in many forms including acute hemolytic transfusion reaction, allergic reaction and transfusion-related acute lung injury. We previously developed an acute hemolytic transfusion reaction rat model mediated by transfusion of incompatible human erythrocytes against which rats have preexisting antibodies resulting in classical complement pathway mediated intravascular hemolysis. In this study, the acute hemolytic transfusion reaction model was adapted to yield an acute lung injury phenotype. Adolescent male Wistar rats were primed in the presence or absence of lipopolysaccharide followed by transfusion of incompatible erythrocytes. Blood was collected at various time points during the course of the experiment to determine complement C5a levels and free

SUBMITTER: Gregory Rivera M 

PROVIDER: S-EPMC7170260 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature