The ABC exporter IrtAB imports and reduces mycobacterial siderophores.

ABSTRACT: Intracellular replication of the deadly pathogen Mycobacterium tuberculosis relies on the production of small organic molecules called siderophores that scavenge iron from host proteins¹. M. tuberculosis produces two classes of siderophore, lipid-bound mycobactin and water-soluble carboxymycobactin^2,3. Functional studies have revealed that iron-loaded carboxymycobactin is imported into the cytoplasm by the ATP binding cassette (ABC) transporter IrtAB⁴, which features an additional cytoplasmic siderophore interaction domain⁵. However, the predicted ABC exporter fold of IrtAB is seemingly contradictory to its import function. Here we show that membrane-reconstituted IrtAB is sufficient to import mycobactins, which are then reduced by the siderophor

SUBMITTER: Arnold FM 

PROVIDER: S-EPMC7170716 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature