Enhanced noninvasive imaging of oncology models using the NIS reporter gene and bioluminescence imaging.

ABSTRACT: Noninvasive bioluminescence imaging (BLI) of luciferase-expressing tumor cells has advanced pre-clinical evaluation of cancer therapies. Yet despite its successes, BLI is limited by poor spatial resolution and signal penetration, making it unusable for deep tissue or large animal imaging and preventing precise anatomical localization or signal quantification. To refine pre-clinical BLI methods and circumvent these limitations, we compared and ultimately combined BLI with tomographic, quantitative imaging of the sodium iodide symporter (NIS). To this end, we generated tumor cell lines expressing luciferase, NIS, or both reporters, and established tumor models in mice. BLI provided sensitive early detection of tumors and relatively easy monitoring of disease progression. However, spatial res

SUBMITTER: Vandergaast R 

PROVIDER: S-EPMC7170803 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature