Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley.

ABSTRACT:

Key message

The Taguchi method and metAFLP analysis were used to optimise barley regenerants towards maximum and minimum levels of tissue culture-induced variation. The subtle effects of symmetric and asymmetric methylation changes in regenerants were identified. Plant tissue cultures (PTCs) provide researchers with unique materials that accelerate the development of new breeding cultivars and facilitate studies on off-type regenerants. The emerging variability of regenerants derived from PTCs may have both genetic and epigenetic origins, and may be desirable or degrade the value of regenerated plants. Thus, it is crucial to determine how the PTC variation level can be controlled. The easiest way to manipulate total tissue culture-induced variation (TTCIV) is to utilise appropriate

SUBMITTER: Orlowska R 

PROVIDER: S-EPMC7170832 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature