Structural basis of astrocytic Ca²⁺ signals at tripartite synapses.

ABSTRACT: Astrocytic Ca²⁺ signals can be fast and local, supporting the idea that astrocytes have the ability to regulate single synapses. However, the anatomical basis of such specific signaling remains unclear, owing to difficulties in resolving the spongiform domain of astrocytes where most tripartite synapses are located. Using 3D-STED microscopy in living organotypic brain slices, we imaged the spongiform domain of astrocytes and observed a reticular meshwork of nodes and shafts that often formed loop-like structures. These anatomical features were also observed in acute hippocampal slices and in barrel cortex in vivo. The majority of dendritic spines were contacted by nodes and their sizes were correlated. FRAP experiments and Ca²⁺ imaging showed that nodes were biochemic

SUBMITTER: Arizono M 

PROVIDER: S-EPMC7170846 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature