Parentage and relatedness reconstruction in Pinus sylvestris using genotyping-by-sequencing.

ABSTRACT: Estimating kinship is fundamental for studies of evolution, conservation, and breeding. Genotyping-by-sequencing (GBS) and other restriction based genotyping methods have become widely applied in these applications in non-model organisms. However, sequencing errors, depth, and reproducibility between library preps could potentially hinder accurate genetic inferences. In this study, we tested different sets of parameters in data filtering, different reference populations and eight estimation methods to obtain a robust procedure for relatedness estimation in Scots pine (Pinus sylvestris L.). We used a seed orchard as our study system, where candidate parents are known and pedigree reconstruction can be compared with theoretical expectations. We found that relatedness estimates were lower tha

SUBMITTER: Hall D 

PROVIDER: S-EPMC7171117 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature