INOS-inhibitor driven neuroprotection in a porcine retina organ culture model.

ABSTRACT: Nitrite oxide plays an important role in the pathogenesis of various retinal diseases, especially when hypoxic processes are involved. This degeneration can be simulated by incubating porcine retinal explants with CoCl₂ . Here, the therapeutic potential of iNOS-inhibitor 1400W was evaluated. Degeneration through CoCl₂ and treatment with the 1400W were applied simultaneously to porcine retinae explants. Three groups were compared: control, CoCl₂ , and CoCl₂  + iNOS-inhibitor (1400W). At days 4 and 8, retinal ganglion cells (RGCs), bipolar, and amacrine cells were analysed. Furthermore, the influence on the glia cells and different stress markers were evaluated. Treatment with CoCl₂ resulted in a significant loss of RGCs already after 4 

SUBMITTER: Hurst J 

PROVIDER: S-EPMC7171393 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature