Protection of the transplant kidney during cold perfusion with doxycycline: proteomic analysis in a rat model.

ABSTRACT:

Background

It has been previously shown that doxycycline (Doxy) protects the kidney from preservation injury by inhibition of matrix metalloproteinase. However, the precise molecular mechanism involved in this protection from injury is not known. We used a pharmaco-proteomics approach to identify potential molecular targets associated with kidney preservation injury.

Methods

Rat kidneys were cold perfused with or without doxycycline (Doxy) for 22 h. Kidneys perfusates were analyzed for the presence of injury markers such as lactate dehydrogenase (LDH), and neutrophil-gelatinase associated lipocalin (NGAL). Proteins extracted from kidney tissue were analyzed by 2-dimensional gel electrophoresis. Proteins of interest were identified by mass spectrometry.

Results

Triosep

SUBMITTER: Moser MAJ 

PROVIDER: S-EPMC7171734 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature