Lattice complex assembled by noncompetitive anti-EGFR antibodies regulates actin cytoskeletal reorganization.

ABSTRACT:

Background

Recent evidence of clinical trials highlights that the combination of two noncompetitive anti-EGFR antibodies can benefit patients with several cancers. Previous studies propose that a lattice complex assembled by antibodies and EGFR down-regulates surface EGFR by rapid internalization of the complex. However, there remains a paucity of evidence and understanding on the existence of a lattice complex on cell surface and its cellular processes of internalization.

Methods

Herein, we used three dimensions structured illumination microscopy to directly observe the actual morphology of the lattice complex formed on Hela cell membrane after noncompetitive anti-EGFR antibody combinations, and we explored the internalized mechanism of noncompetitive antibody combinations

SUBMITTER: Huang D 

PROVIDER: S-EPMC7171787 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature