Reprint of: Core protein-mediated 5'-3' annealing of the West Nile virus genomic RNA in vitro.

ABSTRACT: Genome cyclization through conserved RNA sequences located in the 5' and 3' terminal regions of flavivirus genomic RNA is essential for virus replication. Although the role of various cis-acting RNA elements in panhandle formation is well characterized, almost nothing is known about the potential contribution of protein cofactors to viral RNA cyclization. Proteins with nucleic acid chaperone activities are encoded by many viruses (e.g., retroviruses, coronaviruses) to facilitate RNA structural rearrangements and RNA-RNA interactions during the viral replicative cycle. Since the core protein of flaviviruses is also endowed with potent RNA chaperone activities, we decided to examine the effect of West Nile virus (WNV) core on 5'-3' genomic RNA annealing in vitro. Core protein binding resulte

SUBMITTER: Ivanyi-Nagy R 

PROVIDER: S-EPMC7172194 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature