Duplex real-time RT-PCR assay for detection and subgroup-specific identification of human respiratory syncytial virus.

ABSTRACT: Human respiratory syncytial virus (HRSV) is a leading cause of acute respiratory illness in young children worldwide. Reliable detection and identification of HRSV subgroup A and B infections are essential for accurate disease burden estimates in anticipation of licensure of novel HRSV vaccines and immunotherapies. To ensure continued reliability, molecular assays must remain current with evolving virus strains. We have developed a HRSV subgroup-specific real-time RT-PCR (rRT-PCR) assay for detection and subgroup identification using primers and subgroup-specific probes targeting a conserved region of the nucleoprotein gene combined in a single duplex reaction using all genome sequence data currently available in GenBank. The assay was validated for analytical sensitivity, specificity, rep

SUBMITTER: Wang L 

PROVIDER: S-EPMC7172218 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature