Reliable quantification of rhinovirus species C using real-time PCR.

ABSTRACT:

Background

Rhinovirus C (RV-C) is an important respiratory pathogen of children, but little is known about its contribution to disease severity, though viral load appears to be important. Difficulty in RV-C cultivation and target sequence variation has precluded the development of a PCR based quantification method.

Objective

The aim of this study was to develop and validate reverse transcription quantitative PCR (RT-qPCR) assays for a broad range of circulating RV-C genotypes in nasopharyngeal aspirates (NPAs).

Study design

Four assays were designed to quantify a 296bp region located within the 5' untranslated region (UTR) of RV-C types. These assays were based on in silico analysis of available RV-C sequences. Probes were designed to provide 100% homology to the corr

SUBMITTER: Sikazwe CT 

PROVIDER: S-EPMC7172306 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature