Gene expression changes in lymphoblastoid cell lines and primary B cells by dexamethasone.

ABSTRACT:

Background

Human Epstein-Barr virus-transformed lymphoblastoid cell lines (LCLs) have been thought to be a useful model system for pharmacogenomics studies. The purpose of this study was to determine the effect of Epstein-Barr virus transformation on gene expression changes by dexamethasone (Dex) in LCLs and primary B cells (PBCs) derived from the same individuals.

Patients and methods

We prepared LCLs and purified PBCs from the same six male donors participating in the Childhood Asthma Management Program clinical trial, and compared mRNA profiles after 6 h incubation with Dex (10 mol/l) or sham buffer. We assessed differential expression and put the list of differentially expressed genes into the web interface of ConsensusPathDB to find the pathway-level interpretation of o

SUBMITTER: Park HW 

PROVIDER: S-EPMC7172499 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature