Cellular DNA quantification in respiratory samples for the normalization of viral load: a real need?

ABSTRACT:

Background

Respiratory tract infections have an enormous social economic impact, with high incidence of hospitalization and high costs. Adequate specimen collection is the first crucial step for the correct diagnosis of viral respiratory infections.

Objectives

The present retrospective study aimed: i) to verify the cell yield obtained from sampling the nasal respiratory tract using mid-turbinate flocked swabs; ii) to evaluate the normalization of viral load, based on cell number; and iii) to compare the kinetics of viral infection obtained with normalized vs non-normalized viral load.

Study design

The number of cells were quantified by real-time PCR in residual extract of nasal swabs tested for respiratory viruses detection and stored at -80 °C in a universal transpor

SUBMITTER: Piralla A 

PROVIDER: S-EPMC7173160 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature