Efficient differentiation and polarization of primary cultured neurons on poly(lactic acid) scaffolds with microgrooved structures.

ABSTRACT: Synthetic biodegradable polymers including poly(lactic acid) (PLA) are attractive cell culture substrates because their surfaces can be micropatterned to support cell adhesion. The cell adhesion properties of a scaffold mainly depend on its surface chemical and structural features; however, it remains unclear how these characteristics affect the growth and differentiation of cultured cells or their gene expression. In this study, we fabricated two differently structured PLA nanosheets: flat and microgrooved. We assessed the growth and differentiation of mouse primary cultured cortical neurons on these two types of nanosheets after pre-coating with poly-D-lysine and vitronectin. Interestingly, prominent neurite bundles were formed along the grooves on the microgrooved nanosheets, whereas th

SUBMITTER: Otomo A 

PROVIDER: S-EPMC7174324 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature