Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells.

ABSTRACT: The nucleus is fundamentally composed by lamina and nuclear membranes that enclose the chromatin, nucleoskeletal components and suspending nucleoplasm. The functional connections of this network integrate external stimuli into cell signals, including physical forces to mechanical responses of the nucleus. Canonically, the morphological characteristics of the nucleus, as shape and size, have served for pathologists to stratify and diagnose cancer patients; however, novel biophysical techniques must exploit physical parameters to improve cancer diagnosis. By using multiple particle tracking (MPT) technique on chromatin granules, we designed a SURF (Speeded Up Robust Features)-based algorithm to study the mechanical properties of isolated nuclei and in living cells. We have determined the app

SUBMITTER: Herraez-Aguilar D 

PROVIDER: S-EPMC7174401 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature