Acrylonitrile-Mediated Nascent RNA Sequencing for Transcriptome-Wide Profiling of Cellular RNA Dynamics.

ABSTRACT: RNA sequencing has greatly facilitated gene expression studies but is weak in studying temporal RNA dynamics; this issue can be addressed by analyzing nascent RNAs. A famous method for nascent RNA analysis is metabolic labeling with noncanonic nucleoside followed by affinity purification, however, purification processes can always introduce biases into data analysis. Here, a chemical method for nascent RNA sequencing that avoids affinity purification based on acrylonitrile-mediated uridine-to-cytidine (U-to-C) conversion (AMUC-seq) via 4-thiouridine (s⁴U) cyanoethylation is presented. This method converts s⁴U base-pairing with guanine through the nucleophilic addition of s⁴U to acrylonitrile. The high reaction efficiency permits AMUC-seq directly and effici

SUBMITTER: Chen Y 

PROVIDER: S-EPMC7175251 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature