The Effects of Lactose Induction on a Plasmid-Free E. coli T7 Expression System.

ABSTRACT: Recombinant production of pharmaceutical proteins like antigen binding fragments (Fabs) in the commonly-used production host Escherichia coli presents several challenges. The predominantly-used plasmid-based expression systems exhibit the drawback of either excessive plasmid amplification or plasmid loss over prolonged cultivations. To improve production, efforts are made to establish plasmid-free expression, ensuring more stable process conditions. Another strategy to stabilize production processes is lactose induction, leading to increased soluble product formation and cell fitness, as shown in several studies performed with plasmid-based expression systems. Within this study we wanted to investigate lactose induction for a strain with a genome-integrated gene of interest for the first t

SUBMITTER: Hausjell J 

PROVIDER: S-EPMC7175309 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature