A Novel Fluorescent Clinical Method to Rapidly Quantify Plasma Volume.

ABSTRACT:

Objectives

To determine the performance of a rapid fluorescent indicator technique for measuring plasma volume (PV).

Methods

This was an open-label, observational evaluation of a two-component intravenous visible fluorescent dye technique to rapidly measure PV in 16 healthy subjects and 16 subjects with chronic kidney disease (8 stage 3 and 8 stage 4 CKD), at 2 clinical research sites. The method consisted of a single intravenous injection of 12 mg of a large 150-kDa carboxy-methyl dextran conjugated to a fluorescent rhodamine-derived dye as the PV marker (PVM), and 35 mg of a small 5-kDa carboxy-methyl dextran conjugated to fluorescein, the renal clearance marker. Dye concentrations were quantified 15 min after the injections for initial PV measurements using the indicator-

SUBMITTER: Molitoris BA 

PROVIDER: S-EPMC7175413 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature