Myosin II Filament Dynamics in Actin Networks Revealed with Interferometric Scattering Microscopy.

ABSTRACT: The plasma membrane and the underlying cytoskeletal cortex constitute active platforms for a variety of cellular processes. Recent work has shown that the remodeling acto-myosin network modifies local membrane organization, but the molecular details are only partly understood because of difficulties with experimentally accessing the relevant time and length scales. Here, we use interferometric scattering microscopy to investigate a minimal acto-myosin network linked to a supported lipid bilayer membrane. Using the magnitude of the interferometric contrast, which is proportional to molecular mass, and fast acquisition rates, we detect and image individual membrane-attached actin filaments diffusing within the acto-myosin network and follow individual myosin II filament dynamics. We quantify

SUBMITTER: Mosby LS 

PROVIDER: S-EPMC7175421 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature