BAMscale: quantification of next-generation sequencing peaks and generation of scaled coverage tracks.

ABSTRACT:

Background

Next-generation sequencing allows genome-wide analysis of changes in chromatin states and gene expression. Data analysis of these increasingly used methods either requires multiple analysis steps, or extensive computational time. We sought to develop a tool for rapid quantification of sequencing peaks from diverse experimental sources and an efficient method to produce coverage tracks for accurate visualization that can be intuitively displayed and interpreted by experimentalists with minimal bioinformatics background. We demonstrate its strength and usability by integrating data from several types of sequencing approaches.

Results

We have developed BAMscale, a one-step tool that processes a wide set of sequencing datasets. To demonstrate the usefulness of BAMscal

SUBMITTER: Pongor LS 

PROVIDER: S-EPMC7175505 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature