An efficient in vitro regeneration system from different wild apple (Malus sieversii) explants.

ABSTRACT:

Background

Wild apple, Malus sieversii, is an endangered species and a valuable genetic resource that requires a variety of conservation techniques. This study aimed to investigate the influence of different concentrations of hormones on wild apple regeneration from leaf and stem explants to establish an optimal regeneration system.

Results

Leaves and stems derived from seedlings were cultured on several media supplemented with various concentrations of thidiazuron (TDZ) or 6-benzylaminopurine (BA) in different combinations with 1-naphthaleneacetic acid (NAA). The results showed that the most efficient shoot formation media (35% and 90%) were MS medium containing 4.0 mg L^-1 TDZ and 1.0 mg L^-1 NAA for leaf explants and MS medium containing 1.0 mg

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7175559 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature