Matrix-Matched Calibration Curves for Assessing Analytical Figures of Merit in Quantitative Proteomics.

ABSTRACT: Mass spectrometry is a powerful tool for quantifying protein abundance in complex samples. Advances in sample preparation and the development of data-independent acquisition (DIA) mass spectrometry approaches have increased the number of peptides and proteins measured per sample. Here, we present a series of experiments demonstrating how to assess whether a peptide measurement is quantitative by mass spectrometry. Our results demonstrate that increasing the number of detected peptides in a proteomics experiment does not necessarily result in increased numbers of peptides that can be measured quantitatively.

SUBMITTER: Pino LK 

PROVIDER: S-EPMC7175947 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature