Endosome motility defects revealed at super-resolution in live cells using HIDE probes.

ABSTRACT: We report new lipid-based, high-density, environmentally sensitive (HIDE) probes that accurately and selectively image endo-lysosomes and their dynamics at super-resolution for extended times. Treatment of live cells with the small molecules DiIC₁₆TCO or DiIC_16'TCO followed by in situ tetrazine ligation reaction with the silicon-rhodamine dye SiR-Tz generates the HIDE probes DiIC₁₆-SiR and DiIC_16'-SiR in the endo-lysosomal membrane. These new probes support the acquisition of super-resolution videos of organelle dynamics in primary cells for more than 7 min with no detectable change in endosome structure or function. Using DiIC₁₆-SiR and DiIC_16'-SiR, we describe direct evidence of endosome motility defects in cells from pati

SUBMITTER: Gupta A 

PROVIDER: S-EPMC7176048 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature