Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design.

ABSTRACT: The process by which a zygote develops from a single cell into a multicellular organism is poorly understood. Advances are hindered by detection specificity and sensitivity limitations of single-cell protein tools and by challenges in integrating multimodal data. We introduce an open microfluidic tool expressly designed for same-cell phenotypic, protein, and mRNA profiling. We examine difficult-to-study-yet critically important-murine preimplantation embryo stages. In blastomeres dissociated from less well-studied two-cell embryos, we observe no significant GADD45a protein expression heterogeneity, apparent at the four-cell stage. In oocytes, we detect differences in full-length versus truncated DICER-1 mRNA and protein, which are insignificant by the two-cell stage. Single-embryo analyses

SUBMITTER: Rosas-Canyelles E 

PROVIDER: S-EPMC7176412 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature