An ultraportable and versatile point-of-care DNA testing platform.

ABSTRACT: Point-of-care testing (POCT) has broad applications in resource-limited settings. Here, a POCT platform termed POCKET (point-of-care kit for the entire test) is demonstrated that is ultraportable and versatile for analyzing multiple types of DNA in different fields in a sample-to-answer manner. The POCKET is less than 100 g and smaller than 25 cm in length. The kit consists of an integrated chip (i-chip) and a foldable box (f-box). The i-chip integrates the sample preparation with a previously unidentified, triple signal amplification. The f-box uses a smartphone as a heater, a signal detector, and a result readout. We detected different types of DNA from clinics to environment to food to agriculture. The detection is sensitive (<10³ copies/ml), specific (single-base differentia

SUBMITTER: Xu H 

PROVIDER: S-EPMC7176422 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature