Profiling of subcellular EGFR interactome reveals hnRNP A3 modulates nuclear EGFR localization.

ABSTRACT: The aberrant subcellular translocation and distribution of epidermal growth factor receptor (EGFR) represent a major yet currently underappreciated cancer development mechanism in non-small cell lung cancer (NSCLC). In this study, we investigated the subcellular interactome of EGFR by using a spectral counting-based approach combined with liquid chromatography-tandem mass spectrometry to understand the associated protein networks involved in the tumorigenesis of NSCLC. A total of 54, 77, and 63 EGFR-interacting proteins were identified specifically in the cytosolic, mitochondrial, and nuclear fractions from a NSCLC cell line, respectively. Pathway analyses of these proteins using the KEGG database shown that the EGFR-interacting proteins of the cytosol and nucleus are involved in the ribos

SUBMITTER: Wang TH 

PROVIDER: S-EPMC7176650 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature