Chronic Intermittent Hypoxia Triggers a Senescence-like Phenotype in Human White Preadipocytes.

ABSTRACT: Obstructive sleep apnea (OSA) is a common sleep disorder associated with obesity. Emerging evidence suggest that OSA increases the risk of cardiovascular morbidity and mortality partly via accelerating the process of cellular aging. Thus, we sought to examine the effects of intermittent hypoxia (IH), a hallmark of OSA, on senescence in human white preadipocytes. We demonstrate that chronic IH is associated with an increased generation of mitochondrial reactive oxygen species along with increased prevalence of cells with nuclear localization of ?H2AX & p16. A higher prevalence of cells positive for senescence-associated ?-galactosidase activity was also evident with chronic IH exposure. Intervention with aspirin, atorvastatin or renin-angiotensin system (RAS) inhibitors effectively attenuated IH-mediated senescence-like phenotype. Importantly, the validity of in vitro findings was confirmed by examination of the subcutaneous abdominal adipose tissue which showed that OSA patients had a significantly higher percentage of cells with nuclear localization of ?H2AX & p16 than non-OSA individuals (20.1?±?10.8% vs. 10.3?±?2.7%, Padjusted?

SUBMITTER: Polonis K 

PROVIDER: S-EPMC7176724 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature