ADP exerts P2Y₁₂ -dependent and P2Y₁₂ -independent effects on primary human T cell responses to stimulation.

ABSTRACT: Purinergic signaling plays a complex role in inflammation. Nucleotides released by T lymphocytes, endothelial cells, and platelets during inflammation induce cellular responses by binding to receptors that regulate intracellular signaling pathways. Previous studies have found that purinergic signaling can have both proinflammatory and anti-inflammatory effects, but the roles of specific pathways in specific cell types are poorly understood. We investigated the role of the P2Y₁₂ signaling pathway in the activation of T lymphocytes in vitro. We isolated peripheral blood mononuclear cells (PBMCs) from healthy donors and pretreated them with ADP (a P2Y₁₂ agonist), AR-C69931MX (a P2Y₁₂ antagonist), or both. We then stimulated PBMC using phytohemagglutinin (PHA)

SUBMITTER: Vemulapalli H 

PROVIDER: S-EPMC7176780 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature