TRA1: A Locus Responsible for Controlling Agrobacterium-Mediated Transformability in Barley.

ABSTRACT: In barley (Hordeum vulgare L.), Agrobacterium-mediated transformation efficiency is highly dependent on genotype with very few cultivars being amenable to transformation. Golden Promise is the cultivar most widely used for barley transformation and developing embryos are the most common donor tissue. We tested whether barley mutants with abnormally large embryos were more or less amenable to transformation and discovered that mutant M1460 had a transformation efficiency similar to that of Golden Promise. The large-embryo phenotype of M1460 is due to mutation at the LYS3 locus. There are three other barley lines with independent mutations at the same LYS3 locus, and one of these, Risø1508 has an identical missense mutation to that in M1460. However, none of the <

SUBMITTER: Orman-Ligeza B 

PROVIDER: S-EPMC7176908 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature