Evaluation of a fully automated bioinformatics tool to predict antibiotic resistance from MRSA genomes.

ABSTRACT:

Objectives

The genetic prediction of phenotypic antibiotic resistance based on analysis of WGS data is becoming increasingly feasible, but a major barrier to its introduction into routine use is the lack of fully automated interpretation tools. Here, we report the findings of a large evaluation of the Next Gen Diagnostics (NGD) automated bioinformatics analysis tool to predict the phenotypic resistance of MRSA.

Methods

MRSA-positive patients were identified in a clinical microbiology laboratory in England between January and November 2018. One MRSA isolate per patient together with all blood culture isolates (total n = 778) were sequenced on the Illumina MiniSeq instrument in batches of 21 clinical MRSA isolates and three controls.

Results

The NGD system activated pos

SUBMITTER: Kumar N 

PROVIDER: S-EPMC7177496 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature