The Position and Complex Genomic Architecture of Plant T-DNA Insertions Revealed by 4SEE.

ABSTRACT: The integration of T-DNA in plant genomes is widely used for basic research and agriculture. The high heterogeneity in the number of integration events per genome, their configuration, and their impact on genome integrity highlight the critical need to detect the genomic locations of T-DNA insertions and their associated chromosomal rearrangements, and the great challenge in doing so. Here, we present 4SEE, a circular chromosome conformation capture (4C)-based method for robust, rapid, and cost-efficient detection of the entire scope of T-DNA locations. Moreover, by measuring the chromosomal architecture of the plant genome flanking the T-DNA insertions, 4SEE outlines their associated complex chromosomal aberrations. Applying 4SEE to a collection of confirmed T-DNA lines revealed previousl

SUBMITTER: Krispil R 

PROVIDER: S-EPMC7177604 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature