MiR-1285-3p Controls Colorectal Cancer Proliferation and Escape from Apoptosis through DAPK2.

ABSTRACT: MicroRNAs are tiny but powerful regulators of gene expression at the post-transcriptional level. Aberrant expression of oncogenic and tumor-suppressor microRNAs has been recognized as a common feature of human cancers. Colorectal cancer represents a major clinical challenge in the developed world and the design of innovative therapeutic approaches relies on the identification of novel biological targets. Here, we perform a functional screening in colorectal cancer cells using a library of locked nucleic acid (LNA)-modified anti-miRs in order to unveil putative oncogenic microRNAs whose inhibition yields a cytotoxic effect. We identify miR-1285-3p and further explore the effect of its targeting in both commercial cell lines and primary colorectal cancer stem cells, finding induction of cell

SUBMITTER: Villanova L 

PROVIDER: S-EPMC7177834 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature