Molecular Dissection of TaLTP1 Promoter Reveals Functional Cis-Elements Regulating Epidermis-Specific Expression.

ABSTRACT: Plant epidermis serves important functions in shoot growth, plant defense and lipid metabolism, though mechanisms of related transcriptional regulation are largely unknown. Here, we identified cis-elements specific to shoot epidermis expression by dissecting the promoter of Triticum aestivum lipid transfer protein 1 (TaLTP1). A preliminary promoter deletion analysis revealed that a truncated fragment within 400 bp upstream from the translation start site was sufficient to confer conserved epidermis-specific expression in transgenic Brachypodium distachyon and Arabidopsis thaliana. Further, deletion or mutation of a GC(N4)GGCC motif at position -380 bp caused a loss of expression in pavement cells. With an electrophoretic mobility shift assay (EMSA) and transgeni

SUBMITTER: Wang G 

PROVIDER: S-EPMC7177882 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature