Inorganic Pyrophosphatase-Nanodiamond Conjugates Hydrolyze Pyrophosphate in Human Synovial Fluid.

ABSTRACT: The present work is focused on testing enzyme-based agents for the partial dissolution of calcium pyrophosphate (CaPP_i) deposits in the cartilages and synovial fluid of patients with pyrophosphate arthropathy (CPPD disease). Previously, we suggested that inorganic pyrophosphatases (PPases) immobilized on nanodiamonds of detonation synthesis (NDs) could be appropriate for this purpose. We synthesized and characterized conjugates of NDs and PPases from Escherichia coli and Mycobacterium tuberculosis. The conjugates showed high enzymatic activity and resistance to inhibition by calcium and fluoride. Here, we tested the effectiveness of pyrophosphate (PP_i) hydrolysis by the conjugates in an in vitro model system simulating the ionic composition of the synovi

SUBMITTER: Valueva AV 

PROVIDER: S-EPMC7178341 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature