Culture-induced changes in mRNA expression levels of efflux and SLC-transporters in brain endothelial cells.

ABSTRACT:

Background

The complexity of the neurovascular unit (NVU) poses a challenge in the investigations of drug transport across the blood-brain barrier (BBB) and the function of the brain capillary endothelium. Several in vitro models of the brain capillary endothelium have been developed. In vitro culture of primary endothelial cells has, however, been reported to alter the expression levels of various brain endothelial proteins. Only a limited number of studies have addressed this in detail. The aim of the present study was to investigate mRNA levels of selected BBB transporters and markers in in vitro models of the BBB based on bovine primary endothelial cells and compare these to the levels estimated in freshly isolated bovine brain capillaries.

Methods

Brain capillaries were

SUBMITTER: Goldeman C 

PROVIDER: S-EPMC7178567 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature