Identification of ε-Poly-L-lysine as an Antimicrobial Product from an Epichloe Endophyte and Isolation of Fungal ε-PL Synthetase Gene.

ABSTRACT: The endophytic fungus Epichloë festucae is known to produce bioactive metabolites, which consequently protect the host plants from biotic and abiotic stresses. We previously found that the overexpression of vibA (a gene for transcription factor) in E. festucae strain E437 resulted in the secretion of an unknown fungicide. In the present study, the active substance was purified and chemically identified as ε-poly-L-lysine (ε-PL), which consisted of 28-34 lysine units. The productivity was 3.7-fold compared with that of the wild type strain E437. The isolated ε-PL showed inhibitory activity against the spore germination of the plant pathogens Drechslera erythrospila, Botrytis cinerea, and Phytophthora infestans at 1-10 μg/mL. We also isolated the fun

SUBMITTER: Purev E 

PROVIDER: S-EPMC7179176 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature