Immune response of human cultured cells towards macrocyclic Fe₂PO and Fe₂PC bioactive cyclophane complexes.

ABSTRACT: Synthetic molecules that mimic the function of natural enzymes or molecules have untapped potential for use in the next generation of drugs. Cyclic compounds that contain aromatic rings are macrocyclic cyclophanes, and when they coordinate iron ions are of particular interest due to their antioxidant and biomimetic properties. However, little is known about the molecular responses at the cellular level. This study aims to evaluate the changes in immune gene expression in human cells exposed to the cyclophanes Fe₂PO and Fe₂PC. Confluent human embryonic kidney cells were exposed to either the cyclophane Fe₂PO or Fe₂PC before extraction of RNA. The expression of a panel of innate and adaptive immune genes was analyzed by quantitative real-time PCR.

SUBMITTER: Salazar-Medina AJ 

PROVIDER: S-EPMC7179565 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature